Step 1: Enter sequence information
	Enter sequence below in FASTA format: OR: Enter a filename:

Step 2: Set BLAT parameters

Select target database: druggable GNF clones, human GNF clones, mouse Return: top hit and 5% worse top hit and 10% worse top hit and 20% worse top hit and 30% worse top hit and 40% worse top hit and 50% worse top hit and 60% worse top hit and 70% worse top hit and 80% worse top hit and 90% worse all hits uniqueHit concise psLayout SNPs MinScore (if less than 80% coverage): 10 20 30 50 75 100 (recommended) 200 300 400 500 600 700 800 900 1000 MinIdentity 0 10 20 30 40 50 60 70 80 90 (default) 95 96 97 98 99 99.5 99.9 100 %

BLAT on DNA is designed to quickly find sequences of 90-95% and greater similarity of length 40 bases or more. It may miss more divergent or shorter sequence alignments. It will find perfect sequence matches of 33 bases, and sometimes find them down to 22 bases.

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